Kinda does since blood as his food source means if he can easily move around a century after going without it than he should not be all that effected in a fresh state from fighting and exerting energy to matter in a quick fight.
Uh yeah it does when even a simple fencing match that lasts less than half a minute can make someone start to sweat and tire so why in the hell would Dio moving around and fighting opponents who can match him not do the same by your logic? He most likely has no real stamina issues that matter in a fight against a Nen user since we know that even Netero can eventually tire out as shown in his fight with Meruem. Dio on the other hand has never shown to tire in all his fights so it is doubtful it is a factor for him as much as it is for Netero, and not much of a factor overall since the fight will most likely end sooner than later.
It does if SRSE can do that for him anyway chief.
So still enough to do the job as said by OG who said you need to focus on Ko in order to max out your defense which means Netero's defense is still too weak to defend against SRSE as without Ko, Netero is just somewhere above Killua's Town level defense if he is bloodlusted and trying to go right for the kill before time is frozen.