Ryuko and Satsuki chase after Ragyo at great speeds. This will more or less be straight forward. Go off the official height chart...
1 pixel = 1.64m/32 = 0.05125m
0.05125m X 492 = 25.21500m
T = 1s/24
= 41.6666667ms X 2
= 83.3333334ms
T = 25.21500m/83.3333334ms
= 302.58m/s
Final Results
Ryuko & Satsuki fly after Ragyo = 302.58m/s